Motif analysis of eCLIP data with Homer

Copied from EpiGenie some technological definition:

RIP-chip & RIP-seq: RIP can be coupled to microarray (RIP-chip) or sequencing (RIP-seq) to identify RNAs that are bound by an RBP of interest. Both rely on the specificity of RBP-RNA interaction for immunoprecipitation.

HITS-CLIP (or CLIP-seq): Stands for high-throughput sequencing of RNA isolated by crosslinking immunoprecipitation, which utilizes in vivo UV crosslinking technologies and next-gen sequencing.

PAR-CLIP: Photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation attempts to solve some of the problems of HITS-CLIP, namely, efficiency of crosslinking and resolution of RBP binding sites.

iCLIP: Individual-nucleotide resolution CLIP is a refinement of CLIP that allows single-nucleotide resolution of RBP binding sites.

miCLIP: Methylation individual-nucleotide-resolution crosslinking and immunoprecipitation is a specialized version of iCLIP designed to determine which RNA nucleotides are methylated by the RNA methyltransferase Nsun2.

And also eCLIP:

eCLIP maps the binding sites of RBPs on their target RNAs using a modified individual nucleotide resolution CLIP (iCLIP) protocol, improving efficiency and decreasing execution complexity. The hallmark of this method is the ligation of barcoded single-stranded DNA adapters, which reduce amplification bias significantly.

https://www.illumina.com/science/sequencing-method-explorer/kits-and-arrays/eclip.html